Table II. AMP-PNP affinity and ATPase activity of the A107N mutant.

Construct	Kd AMP-PNP-Mg2+ (µM)	ATPase activity (mol/min/mol)
		22°C	30°C	37°C	45°C
Wild type	33	0.06	0.23	0.75	2.16
T22I	110	0.35	1.48	3.41	6.42
A107N	37	0.33	1.34	3.81	9.22