Fig. 6. GPI-GnT activity of Lec15/B5 cells transfected with various vectors. (A) GPI-GnT (top panel), Dol-P-Man synthase (middle) and Dol-P-Glu synthase (bottom) activities of Lec15/B5 cells transfected with an empty vector (lane 1), DPM1 (lane 2), DPM2 (lane 3), DPM3 (lane 4) or yeast DPM1 (lane 5). Cell lysates were incubated with radiolabeled UDP-GlcNAc (top), GDP-mannose (middle) or UDP-glucose (bottom). The lipids were analyzed by TLC. The identities of spots are indicated on the left. (B) Restoration of the surface expression of GPI-anchored proteins on Lec15/B5 cells transfected with an empty vector (a), DPM1 (b), DPM2 (c), DPM3 (d) or yeast DPM1 (e).