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. 2010 Nov 17;49(1):125–130. doi: 10.1128/JCM.01401-10

FIG. 1.

FIG. 1.

Limit of detection of cycling probe real-time PCR with control plasmids. FAM fluorescence signals correspond to the oseltamivir-sensitive genotype (His275), and ROX fluorescence signals indicate the oseltamivir-resistant genotype (Tyr275). Control plasmids containing inserts of seasonal H1N1 sequences (sH1N1-OS and sH1N1-OR) reacted with probes sH1N1-His275 and sH1N1-Tyr275, respectively (a and b). Control plasmids harboring H1N1pdm sequences (H1N1pdm-OS and H1N1pdm-OR) reacted with H1N1pdm probes (c and d).