TABLE 2.
Sample type | Virusa | Subtypeb | Susceptibility to oseltamivirc | No. of samples | No. of samples positive with: |
|||
---|---|---|---|---|---|---|---|---|
Seasonal H1N1 probe set |
H1N1pdm probe set |
|||||||
sH1N1-His275 | sH1N1-Tyr275 | H1N1pdm-His275 | H1N1pdm-Tyr275 | |||||
Isolate | Influenza A virus | Seasonal H1N1 | Sensitive | 10 | 10 | 0 | 0 | 0 |
Influenza A virus | Seasonal H1N1 | Resistant | 10 | 0 | 10 | 0 | 0 | |
Influenza A virus | H1N1pdm | Sensitive | 10 | 0 | 0 | 10 | 0 | |
Influenza A virus | H1N1pdm | Resistant | 3 | 0 | 0 | 0 | 3 | |
Influenza A virus | H3N2 | NAd | 10 | 0 | 0 | 0 | 0 | |
Influenza B virus | NA | NA | 10 | 0 | 0 | 0 | 0 | |
Respiratory syncytial virus | NA | NA | 5 | 0 | 0 | 0 | 0 | |
Parainfluenza virus | NA | NA | 1 | 0 | 0 | 0 | 0 | |
Enterovirus | NA | NA | 1 | 0 | 0 | 0 | 0 | |
Rhinovirus | NA | NA | 2 | 0 | 0 | 0 | 0 | |
Adenovirus | NA | NA | 1 | 0 | 0 | 0 | 0 | |
Nasopharyngeal swab | Influenza A virus | Seasonal H1N1 | Sensitive | 15 | 15 | 0 | 0 | 0 |
Influenza A virus | Seasonal H1N1 | Resistant | 15 | 0 | 15 | 0 | 0 | |
Influenza A virus | H1N1pdm | Sensitive | 15 | 0 | 0 | 15 | 0 | |
Influenza A virus | H3N2 | NA | 15 | 0 | 0 | 0 | 0 | |
Influenza B virus | NA | NA | 10 | 0 | 0 | 0 | 0 | |
Respiratory syncytial virus | NA | NA | 5 | 0 | 0 | 0 | 0 | |
Human metapneumovirus virus | NA | NA | 5 | 0 | 0 | 0 | 0 | |
Negative sample | NA | NA | 10 | 0 | 0 | 0 | 0 |
Viruses were initially detected by virus isolation and PCR using specific primers.
Typed and subtyped by hemagglutinin inhibition assay with vaccine strain antisera and PCR using specific primers.
Resistant strains of both subtypes had a histidine-to-tyrosine change in residue 275 of the NA gene.
NA, not addressed.