TABLE 1.
Species and SNP | Cumulative D value | Primera | Primer sequence (5′-3′)b |
---|---|---|---|
E. faecalis | |||
gyd268 | 0.5034 | gyd268GF | GACAAAGAAGTTACTGTTGATGAAGTG |
gyd268AF | GACAAAGAAGTTACTGTTGATGAAGTA | ||
gyd268R | CTACGATATCAGAAGAAACGATTTCG | ||
xpt198c | 0.7536 | xpt198GR | AAATGAATAAACTGAAGCCGTTAAG |
xpt198AR | AAATGAATAAACTGAAGCYGTTAAA | ||
xpt198F | CTTCGCKCGTAAGGCAAAAAGT | ||
aroE355c | 0.8766 | aroE355GR | TGGGATTATAAATAGCATCATACACG |
aroE355AR | TGGGATTATAAATAGCATCATACACA | ||
aroE355F | CCACATGCRCATAGTAGTCCTATAGAAAA | ||
gdh165 | 0.9386 | gdh165AF | CAGCCTATCGTGATGAACCA |
gdh165GF | CAGCCTATCGTGATGAACCG | ||
gdh165R | CGCCAGACCAACGGAAAT | ||
gyd208 | 0.9682 | gyd208AF | GCTCAACGTGTTCCTGTAGCA |
gyd208TF | GCTCAACGTGTTCCTGTAGCT | ||
gyd208R | CCATTACTGCATTYACTTCATCAAC | ||
gki141c | 0.9824 | gki141TR | TTCCCCCGCGCCT |
gki141CR | TTCCCCCGCGCCC | ||
gki141F | TTCCGTTTGCHTTAGATAATGATG | ||
pstS87c | 0.9886 | psts87GR | GACCACTGGTCCCATACCG |
psts87AR | GACCACTGGTCCCATACCA | ||
psts87F | CGTGGATGCCTCTAAATTAGTYGA | ||
pstS390c | 0.992 | pstS390GR | CATCAATGCTTAAGGCAACG |
pstS390AR | CATCAATGCTTAAGGCAACA | ||
pstS390F | CAGTTCGTAAAATTGTTGAACAAACA | ||
E. faecium | |||
pstS452c | 0.3888 | pstS452CR | GTGTACATATGTTCATATGACCAGATTC |
pstS452TR | GTGTACATATGTTCATAKGACCAGATTT | ||
pstS452F | TCGACGGTGTAGAACCAAAAGA | ||
atpA485c | 0.6456 | atpA485CR | CAGCATATGGTGCGATATAAAGC |
atpA485TR | CAGCATATGGTGCGATATAAAGT | ||
atpA485F | ACATTGAAAAAATATGGCGCAAT | ||
gyd160c | 0.7359 | gyd160GR | CCGTCTAATTTACCGTTCAATTCG |
gyd160TR | CCGTCTAATTTACCGTTCAATTCT | ||
gyd160AR | CCGTCTAATTTACCRTTCAATTCA | ||
gyd160F | GCAAACATCGTWCCTAACTCAACW | ||
purK115 | 0.8276 | purK115TF | AGAAAAATCTTTTTTGGAAACGAAT |
purK115CF | RGAAAAATCTTTTTTGGAAACGAAC | ||
purK115R | GATCCCGTCAATCGCATCTT | ||
pstS87 | 0.8782 | pstS87CF | GTGGATCATAAAGTAGCAGTGGTC |
pstS87TF | GTGGATCATAAAGTAGCAGTRGTT | ||
pstS87R | GTAAAGATATCAATCAATTCCTGTTTKG | ||
atpA314 | 0.9122 | atpA314CF | CCGTAAAACAGGGAAAACTTCC |
atpA314TF | CCGTAAAACAGGGAAAACTTCT | ||
atpA314R | GATCATATCTTGRCCTTTTTGGTTRA | ||
atpA188c | 0.9373 | atpA188GR | GTTAACAGATTTACGTTGCATAACG |
atpA188AR | GTTAACAGATTTACGTTGCATAACA | ||
atpA188F | AATYGACGGACTAGGTGAAATCG | ||
purK217c | 0.9509 | purK217AR | CCCTTGCCATCATAGCCA |
purK217GR | CCYTTGCCATCATARCCG | ||
purK217F | GATCGTCAGTCCGACRGATATC |
F, forward primer; R, reverse primer.
The allele-specific primers are indicated with a nucleotide base in boldface at the 3′ end of the sequence. Key to symbols: H = A+T+C, K = G+T, R = A+G, W = A+T, Y = C+T.
The SNP is in the reverse primer.