TABLE 2.
SNP profiles of E. faecalis isolates
| No. of isolates with profile or straina | Polymorphism at SNP: |
SNP profile | ST(s) in MLST | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1, gyd268 | 2, xpt198 | 3, aroE355 | 4, gdh165 | 5, gyd208 | 6, gki141 | 7, pstS87 | 8, pstS390 | |||
| 1 | A | C | C | A | A | G | T | C | ACCAAGTC | ST41, ST146, ST216, ST219, ST239 |
| 2 | A | C | C | A | T | G | T | T | ACCATGTT | ST44, ST189 |
| 1 | A | C | C | G | A | A | T | C | ACCGAATC | ST62, ST85 |
| 1 | A | C | C | G | T | G | T | T | ACCGTGTT | ST113 |
| 1 | A | C | T | A | A | G | T | T | ACTAAGTT | Newb |
| 1 | A | C | T | A | T | G | C | C | ACTATGCC | ST79, ST82 |
| 2 | A | C | T | G | A | A | C | C | ACTGAACC | ST138 |
| 2 | A | C | T | G | T | A | T | C | ACTGTATC | ST40, ST114, ST148, ST198 |
| 1 | A | T | C | A | A | A | C | C | ATCAAACC | ST5, ST21, ST46, ST50, ST70 |
| 1 | A | T | C | A | A | A | C | C | ATCAAACC | ST145, ST152, ST157 |
| 7 | A | T | T | A | A | G | C | T | ATTAAGCT | ST6, ST139, ST181, ST183, ST241 |
| 1 | A | T | T | A | T | G | C | C | ATTATGCC | ST170 |
| 1 | A | T | T | G | A | G | C | T | ATTGAGCT | New |
| 1 | G | C | C | A | A | A | C | C | GCCAAACC | ST186, ST192 |
| 2 | G | C | C | A | T | A | T | T | GCCATATT | ST19, ST20, ST120 |
| 1 | G | C | C | A | T | G | C | C | GCCATGCC | New |
| 1 | G | C | C | G | A | A | T | C | GCCGAATC | ST30, ST56, ST217 |
| 1 | G | C | C | G | T | A | T | T | GCCGTATT | New |
| 17 | G | C | T | G | A | A | C | C | GCTGAACC | ST16, ST66, ST67 |
| 4 | G | C | T | G | A | A | T | C | GCTGAATC | ST26, ST60, ST209, ST214 |
| 1 | G | T | C | G | T | G | T | T | GTCGTGTT | ST36, ST118, ST180 |
| 1 | G | T | T | G | A | A | C | C | GTTGAACC | ST95, ST179 |
| 1 | G | T | T | G | A | G | T | C | GTTGAGTC | ST64, ST101, ST161, ST205 |
| TX2486 | A | T | T | G | A | A | C | T | ATTGAACT | ST2 |
| TX2708 | A | T | T | A | A | G | C | T | ATTAAGCT | ST6 |
| TX0630 | A | T | C | A | T | A | C | T | ATCATACT | ST9 |
| ΔCT value (mean ± SDc) of: | ||||||||||
| A | 6.97 ± 0.37 | NAb | NA | 2.02 ± 0.20 | 7.42 ± 0.54 | 8.88 ± 0.37 | NA | NA | ||
| C | NA | 4.36 ± 0.27 | 3.32 ± 0.47 | NA | NA | NA | 9.77 ± 0.78 | 2.49 ± 0.38 | ||
| G | 3.51 ± 0.38 | NA | NA | 3.69 ± 0.44 | NA | 7.73 ± 0.29 | NA | NA | ||
| T | NA | 2.61 ± 0.18 | 1.48 ± 0.30 | NA | 9.43 ± 0.63 | NA | 9.74 ± 0.92 | 2.2 ± 0.31 | ||
a
Strains were obtained from University of Texas and are fully MLST characterized.
b
New, STs not found in MLST database; NA, not applicable.
c
From pooled results for each polymorphism.