FIG. 1.
Optimization of the PMA-PCR assay for TB drug susceptibility testing. The optimal PMA concentration was examined using cultured M. tuberculosis H37Rv, both heat killed (A) and antibiotic treated (B), followed by DNA extraction and real-time PCR. The data are shown as raw CT or ΔCT (CT control − CT drug), as indicated. (C) Real-time PCR CT was compared to colony counts as a function of incubation time over 1 to 3 days. The efficiency of PMA versus that of EMA was examined and compared by using drug-treated cells (D). *, P < 0.05.