TABLE 2.
Accuracy of PMA-qPCR compared with agar proportion
Antimicrobial agent | PMA-qPCR |
No. of samples with result by agar proportion |
Accuracy (%) | |||
---|---|---|---|---|---|---|
Cutoff ΔCT | 2ΔCT | Result | Susceptible | Resistant | ||
Isoniazid | −1.5 | 0.4 | Susceptible | 11 | 0 | 100 |
Resistant | 0 | 27 | ||||
Rifampin | −2.4 | 0.2 | Susceptible | 11 | 0 | 100 |
Resistant | 0 | 27 | ||||
Ethambutol | −1.4 | 0.4 | Susceptible | 25 | 0 | 89 |
Resistant | 4 | 9 | ||||
Streptomycin | −1.4 | 0.4 | Susceptible | 15 | 1 | 97 |
Resistant | 0 | 22 | ||||
Amikacin | −0.1 | 0.9 | Susceptible | 36 | 0 | 100 |
Resistant | 0 | 2 | ||||
Kanamycin | −0.9 | 0.6 | Susceptible | 33 | 0 | 95 |
Resistant | 2 | 3 | ||||
Capreomycin | −1.5 | 0.4 | Susceptible | 35 | 1 | 95 |
Resistant | 1 | 1 | ||||
Ofloxacin | −2.4 | 0.2 | Susceptible | 32 | 0 | 100 |
Resistant | 0 | 6 | ||||
Moxifloxacin | −0.2 | 0.8 | Susceptible | 34 | 0 | 100 |
Resistant | 0 | 4 | ||||
Ethionamide | −0.7 | 0.6 | Susceptible | 27 | 2 | 92 |
Resistant | 1 | 8 | ||||
p-Aminosalicylic acid | −1.5 | 0.4 | Susceptible | 31 | 0 | 100 |
Resistant | 0 | 7 | ||||
Linezolid | −1.2 | 0.5 | Susceptible | 38 | 0 | 100 |
Resistant | 0 | 0 | ||||
Cycloserine | −1.2 | 0.5 | Susceptible | 38 | 0 | 100 |
Resistant | 0 | 0 | ||||
Pyrazinamidea | −1.2 | 0.5 | Susceptible | 21 | 0 | 92 |
Resistant | 3 | 14 |
MGIT.