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. 2010 Nov 17;49(1):69–75. doi: 10.1128/JCM.01500-10

TABLE 2.

Accuracy of PMA-qPCR compared with agar proportion

Antimicrobial agent PMA-qPCR
No. of samples with result by agar proportion
Accuracy (%)
Cutoff ΔCT 2ΔCT Result Susceptible Resistant
Isoniazid −1.5 0.4 Susceptible 11 0 100
Resistant 0 27
Rifampin −2.4 0.2 Susceptible 11 0 100
Resistant 0 27
Ethambutol −1.4 0.4 Susceptible 25 0 89
Resistant 4 9
Streptomycin −1.4 0.4 Susceptible 15 1 97
Resistant 0 22
Amikacin −0.1 0.9 Susceptible 36 0 100
Resistant 0 2
Kanamycin −0.9 0.6 Susceptible 33 0 95
Resistant 2 3
Capreomycin −1.5 0.4 Susceptible 35 1 95
Resistant 1 1
Ofloxacin −2.4 0.2 Susceptible 32 0 100
Resistant 0 6
Moxifloxacin −0.2 0.8 Susceptible 34 0 100
Resistant 0 4
Ethionamide −0.7 0.6 Susceptible 27 2 92
Resistant 1 8
p-Aminosalicylic acid −1.5 0.4 Susceptible 31 0 100
Resistant 0 7
Linezolid −1.2 0.5 Susceptible 38 0 100
Resistant 0 0
Cycloserine −1.2 0.5 Susceptible 38 0 100
Resistant 0 0
Pyrazinamidea −1.2 0.5 Susceptible 21 0 92
Resistant 3 14
a

MGIT.