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. 2010 Nov 3;49(1):345–353. doi: 10.1128/JCM.00936-10

FIG. 1.

FIG. 1.

Determining the base composition from mass spectral data: example using BCB115. (A) The spectra are collected and displayed as a 96-well plate format. Mass spectra can be viewed independently for each of the 96 wells. (B) Electrospray-generated mass spectra. This sample contains the PCR amplicons from two different organisms: Bacteroides thetaiotaomicron (top) and Staphylococcus aureus (bottom). (C) Deconvoluted mass spectra showing molecular masses of the forward and reverse strands of each PCR amplicon. An algorithm is used to calculate the base composition from the molecular mass and was calculated to be 30A-27G-23C-19T (top) and 27A-30G-21C-21T (bottom).