TABLE 4.

Characteristics of potentially functional IS elements detected in L. pneumophila Paris^c

Name	Family	Subgroup	Gene for ORF1	Gene for ORF2	End	IR (bp)	DR (bp)	Length (bp)	Copy no.
									Paris	Philadelphia	Lens	Corby
ISLpn1	IS4	IS10	lpp0125	No	CT	16	0	1,355	2	0	0	0
ISLpn8	IS3	IS407	lpp0199	lpp0200	TG	32	0	1,199	2	2	1	0
ISLpn9	IS4	IS10	lpp0038	lpp0039	CT	60	9	1,628	7 (+2)a	1	0	0
ISLpn10	IS5	IS427	lpp0083	No	GG	22	2	896	2	0	0	0
ISLpn11b	ISL3		lpp2037	No	GG	20	8	1,326	6	0 (+1)a	0	1
ISLpn12	IS21		lpp1563	lpp1564	TG	41	7	1,996	2	0	0	1

^aIn some cases, truncated copies of some IS elements are present, and their numbers are indicated in parentheses.

^bWhile one of the six ISLpn11 copies in L. pneumophila Paris presented no duplication of the target site, the five others led to 8-bp duplications of the insertion target, with no obvious similarity between them (data not shown). Moreover, the insertion sites are widely distributed on the Paris chromosome, even if two insertions occurred close to tRNA-encoding genes, tRNALys and tRNAMet. In the Philadelphia strain, the unique ISLpn11 copy is also localized close to the tRNAMet-encoding gene.

^cFor each IS element, the following features are given: family and subgroup, name of the genes encoding ORF1 and ORF2, last two nucleotides (End), length of IRs, length of the direct repeats (DR) generated upon insertion of the IS, total length, and copy number in the genome of the four strains Paris, Lens (5), Philadelphia (7), and Corby (25).