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MHV RNA synthesis in CB3 (hnRNP A1-deficient) and CB3A (hnRNP A1-restored) cells. Viral RNA was metabolically labeled with [3H]uridine, in the presence of actinomycin D, in CB3 and CB3A cells that were mock-infected or infected with wild-type MHV (wt), the win mutant, or the igs4-win mutant; mock-infected and wild-type MHV-infected mouse L2 cells were included for comparison. RNA was separated by agarose gel electrophoresis and visualized by fluorography.
