Skip to main content
. 2010 Nov 24;85(3):1322–1329. doi: 10.1128/JVI.01367-10

FIG. 2.

FIG. 2.

Expression of viral components and GFP in the 293T cells transfected with plasmid DNAs. 293T cells transfected with the 10 (WT) or 9 (ΔCM2) plasmid DNAs were analyzed (see Materials and Methods for plasmid composition). (A) The lysates of the 293T cells at 24 h p.t. were electrophoresed and subjected to immunoblotting using a mixture of MAbs against HEF, NP, and M1 (left) and with antisera against CM2, NS1, and NS2 (right). (B) The plasmid-transfected 293T cells were observed using a fluorescent microscope. The mock-transfected cells are shown in the left panel (magnification, ×50). (C) RNA was extracted from the 293T cells, treated with DNase, reverse transcribed, and then subjected to real-time PCR for quantification of GFP-vRNA. The relative copy number of the GFP-vRNA from 293T cells transfected with the 10 plasmids (WT) is expressed as 1.0. The result from mock-transfected cells was shown as the control. A representative result is shown.