FIG. 5.

An HIV-1 protease-deficient provirus restores the expression of RIG-I. HEK293 cells were transfected with a plasmid expressing Myc-RIG-I. At 24 h posttransfection cells were transfected with either the WT BH10 proviral clone or the BH10-protease-defective proviral clone. A GFP-expressing plasmid alone was used as an internal control. Whole-cell extracts were subjected to Western blot analysis using anti-Myc, anti-GFP, anti-IRF-3, anti-p24 Gag, and anti-β-actin antibodies. The 55-kDa band represents the immature Gag polyprotein, whereas the 24-kDa band represents the mature Gag protein.