TABLE 2.
Recovery of infectious viruses using different IBDV segment A constructsa
| Construct name | Description or change in segment A | Recovery resultb |
|---|---|---|
| pIBDVA | Strain D78 segment A | + |
| pIBDV-1 | Replacement of 5′ terminus of VP5 coding sequence (nt 100-129) with c-Myc sequence | − |
| pIBDV-2 | Replacement of 5′ terminus of VP2 coding sequence (nt 134-163) with c-Myc sequence | − |
| pIBDV-3 | Insertion of c-Myc sequence at 5′ terminus of VP5 coding sequence (between nt 99 and 100) | + |
| pIBDV-4 | Insertion of c-Myc sequence at 5′ terminus of VP2 coding sequence (between nt 133 and 134) | − |
| pIBDV-5 | Insertion of HCV(523-535) epitope at 5′ terminus of VP5 coding sequence (between nt 99 and 100) | + |
| pIBDV-6 | Insertion of HCV(412-419) epitope at 5′ terminus of VP5 coding sequence (between nt 99 and 100) | + |
| pIBDV-7 | Mutation and insertion of FLAG epitope in PBC loop of VP2 (corresponding to nt 794-800) | − |
| pIBDV-8 | Mutation and insertion of HCV(523-535) epitope in PBC loop of VP2 (corresponding to nt 794-800) | − |
| pIBDV-9 | Substitution of FLAG epitope in PHI loop of VP2 (corresponding to nt 1076-1100) | − |
| pIBDV-10 | Substitution of HCV(412-419) epitope in PHI loop of VP2 (corresponding to nt 1076-1100) | − |
a
Amino acid sequences of epitopes were as follows: c-Myc epitope, EQKLISEEDL; FLAG epitope, DYKDDDDK; HCV(523-535) epitope, GAPTYSWGANDTDV; and HCV(412-419) epitope, QLINTNGS. Vero cells were transfected with various IBDV segment A constructs, plus segment B.
b
Results for recovery of virus are denoted as + (virus was recovered) and − (no virus was recovered).