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. 2010 Nov 3;85(3):1193–1204. doi: 10.1128/JVI.01889-10

FIG. 6.

FIG. 6.

NS3 Q221L suppresses the assembly defect of full-length genomes containing the NS4A K41A mutation. (A) Replication phenotypes of NS4A K41A and variants containing NS3 Q221L or NS4B S230P. Huh-7.5 cells were transfected with each indicated mutant, and media were collected at 24, 48, 72, and 96 h posttransfection. Values represent the time course of secreted GLuc activity for each transfection, expressed as an average of at least three independent transfections. Error bars represent the standard deviation of the mean. (B) Relative infectivity phenotypes of NS4A K41A and variants containing NS3 Q221L or NS4B S230P. The levels of infectivity levels in the media from panel A were quantified by infecting naive cells and monitoring secreted GLuc activity. Values represent the time course of secreted GLuc activity for each transfection, expressed as an average of at least three independent transfections. Error bars represent the standard deviation of the mean.