FIG. 1.

Mus pahari cells express functional receptor for XMRV. (A) Detection of XMRV proteins in primary Mus pahari cells by immunostaining. XMRV Gag and Env proteins (green) were detected by goat anti-MLV-p30/gp70 polyclonal antibodies. Cells and nuclei were counterstained with anti-β-actin antibody (red) and DAPI (blue), respectively. (B) Production of infectious XMRV virions from XMRV-infected Mus pahari cells. 293T cells were infected with the supernatants harvested from XMRV-infected and uninfected Mus pahari cells at 1 and 5 days after infection. XMRV-specific proviral DNA copy numbers in 293T cells were determined by real-time PCR as described in Materials and Methods.