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. 2010 Nov 17;85(3):1174–1181. doi: 10.1128/JVI.01578-10

FIG. 5.

FIG. 5.

Comparative Western blot (WB) analysis of the proteinase K-resistant fragment (PrPSc) of the prion protein. Discrimination between BSE and natural scrapie is achieved using two monoclonal antibodies, 6H4 (a) and 12B2 (b). Lane 1, 4.2 mg equivalent of brain material (mgE) of natural scrapie isolate from the NPU flock. Lane 2, 20 mg equivalent of inocula NPU J2501, Cheviot sheep experimentally infected via the oral route with cattle BSE. Lanes 3 and 5, 1.2 and 1.5 mg equivalent of 129/Ola mice infected with a natural scrapie isolate. Lane 4, 64 mg equivalent of HuMM transgenic mouse infected with experimental sheep BSE (inoculum 2). Lanes 6 and 7, 2.8 and 0.88 mg equivalent of 129/Ola and Bov6 (respectively) infected with experimental sheep BSE (inoculum 2). Lane 8, 0.6 mg equivalent of ME7/SV control. Molecular markers (M) of the standards are indicated on either side of the panels (in kDa).