TABLE 2.
Primer pairs for sequencing rtxA1 in various strains
| CECT 4999 (C), A14 (P), and 95-8-161 (P)a |
CECT 529T |
11028 |
94-9-119 |
Riu-1 |
|||||
|---|---|---|---|---|---|---|---|---|---|
| Primer | Sequence (5′-3′) | Primer | Sequence (5′-3′) | Primer | Sequence (5′-3′) | Primer | Sequence (5′-3′) | Primer | Sequence (5′-3′) |
| 0F | GTGACGCTCTTTGGTGCTC | 0F | GTGACGCTCTTTGGTGCTC | 0F | GTGACGCTCTTTGGTGCTC | 0F | GTGACGCTCTTTGGTGCTC | 0F | GTGACGCTCTTTGGTGCTC |
| 0R | CAGTCGTTTGTCTTTGGTG | 0R | CAGTCGTTTGTCTTTGGTG | 0R | CAGTCGTTTGTCTTTGGTG | 0R | CAGTCGTTTGTCTTTGGTG | 0R | CAGTCGTTTGTCTTTGGTG |
| 1F | CCACACGGTTGAAGTTGCCA | 1F | CCACACGGTTGAAGTTGCCA | 1F | CCACACGGTTGAAGTTGCCA | 1F | CCACACGGTTGAAGTTGCCA | 1F | CCACACGGTTGAAGTTGCCA |
| 1R | ACGCCAGAAGATTTGCCTCGT | 1R | ACGCCAGAAGATTTGCCTCGT | 1R | ACGCCAGAAGATTTGCCTCGT | 1R | ACGCCAGAAGATTTGCCTCGT | 1R | ACGCCAGAAGATTTGCCTCGT |
| 2F | GACGAGGCAAATCTTCTGGCG | 2F | GACGAGGCAAATCTTCTGGCG | 2F | GACGAGGCAAATCTTCTGGCG | 2F | GACGAGGCAAATCTTCTGGCG | 2F | GACGAGGCAAATCTTCTGGCG |
| 2R | GATGGATGCGAATGGTCTT | 2R | GATGGATGCGAATGGTCTT | 2R | GATGGATGCGAATGGTCTT | 2R | GATGGATGCGAATGGTCTT | 2R | GATGGATGCGAATGGTCTT |
| 3F_Bt2 | CGTCTACAGCCAGTTCAG | 3F_Bt1 | GATGTCGCCTTCCGCAATACC | 3F_Bt1 | GATGTCGCCTTCCGCAATACC | 3F_Bt1 | GATGTCGCCTTCCGCAATACC | 3F_Bt2 | CGTCTACAGCCAGTTCAG |
| 3R_Bt2 | GGTGGATGCGAAAGCGACT | 3R_Bt2 | GGTGGATGCGAAAGCGACT | 5R_Bt1 | CTGAGCAATCCAACCCTTTAC | 3R_Bt1 | GGAGTCACAGTCATTGGC | 3R_Bt2 | GGTGGATGCGAAAGCGACT |
| 4F_Bt2 | CTGTCTGAGTAAAGCGTTGG | 4F_Bt2 | CTGTCTGAGTAAAGCGTTGG | 6F_Bt1 | CCTGCCACACTTTCATC | 4F2_Bt1 | AATGTTGTTCGCAGCACCAG | 4F_Bt2 | CTGTCTGAGTAAAGCGTTGG |
| 4R_Bt2 | CAACCTGCGTGGCTATGG | 6R_Bt1 | GCGGTGTCTGGCTTATT | 6R_Bt1 | GCGGTGTCTGGCTTATT | 4R2_Bt1 | CAGGGCGTAATCAAGCG | 4R_Bt2 | CAACCTGCGTGGCTATGG |
| 5F_Bt2 | CGCTTTGGTCATACTTGGC | 7F_Bt1 | CGTTAGCAGCAGAATCGGCGT | 7F_Bt1 | CGTTAGCAGCAGAATCGGCGT | 5F_Bt1 | CGCATCAAAACCAAGGTCAC | 5F_Riu1 | CGGTCAAGCAATAAGCCAGA |
| 5R_Bt2 | ATGCGAAGAACCCAATG | 7R_Bt1 | TCTGAAGGCTATGCGAG | 7R_Bt1 | TCTGAAGGCTATGCGAG | 5R_Bt1 | CTGAGCAATCCAACCCTTTAC | 5R_Riu1 | GTATCTGGACGGAAACGG |
| 6F_Bt2 | GGTATCAAGAACATCAGTG | 8F_Bt1 | CTTCATCTGCTTGGCTGGC | 6F_Riu1 | GACAAGGCATCAAGGAG | 6F_Bt1 | CCTGCCACACTTTCATC | 6F_Riu1 | GACAAGGCATCAAGGAG |
| 6R_Bt2 | TGCTCGTCTCATTGCTTTC | 8R_Bt1 | GGATAACAAGGTGCTCTCGC | 8R_Bt1 | GGATAACAAGGTGCTCTCGC | 6R_Bt1 | GCGGTGTCTGGCTTATT | 8F_Bt1 | CTTCATCTGCTTGGCTGGC |
| 7F_Bt2 | GTCTTCCTGTAGTCCTTTC | 8F1_Bt1 | GATACTCCGTCAGGGCGT | 8F1_Bt1 | GATACTCCGTCAGGGCGT | 7F_Bt1 | CGTTAGCAGCAGAATCGGCGT | 6R_Riu1 | GTGGTATGTTCCTTGAC |
| 7R_Bt2 | CCAGTCAAACCACCAAAGC | 8R1_Bt1 | CGATTATGACTGGGACAAACGC | 8R1_Bt1 | CGATTATGACTGGGACAAACGC | 7R_Bt1 | TCTGAAGGCTATGCGAG | 6R2_Riu1 | GCTGGTGGTTTGTTAGA |
| 8F_Bt2 | GCCCATCATCACTCACC | 8F2_Bt1 | CCAGACCAGAAGGAAACAATGC | 8F2_Bt1 | CCAGACCAGAAGGAAACAATGC | 8F_Bt1 | CTTCATCTGCTTGGCTGGC | 9F | GTGTGCGTCAGATTCGG |
| 8R_Bt2 | GTCCGTTGCGAAGTCTGAAGC | 8R2_Bt1 | GAAATCTGAAGCGGGTGTGG | 8R2_Bt1 | GAAATCTGAAGCGGGTGTGG | 8R_Bt1 | GGATAACAAGGTGCTCTCGC | 9R | GAAGAAGTGGGCTCGGAC |
| 9F | GTGTGCGTCAGATTCGG | 9F | GTGTGCGTCAGATTCGG | 9F | GTGTGCGTCAGATTCGG | 8F1_Bt1 | GATACTCCGTCAGGGCGT | 10F | CCTTCACCATTCACGCCATA |
| 9R | GAAGAAGTGGGCTCGGAC | 9R | GAAGAAGTGGGCTCGGAC | 9R | GAAGAAGTGGGCTCGGAC | 8R1_Bt1 | CGATTATGACTGGGACAAACGC | 10R | GGTGTTGTGGCGGCAGC |
| 10F | CCTTCACCATTCACGCCATA | 10F | CCTTCACCATTCACGCCATA | 10F | CCTTCACCATTCACGCCATA | 8F2_Bt1 | CCAGACCAGAAGGAAACAATGC | 11F | CAACAACCCTACCGTGG |
| 10R | GGTGTTGTGGCGGCAGC | 10R | GGTGTTGTGGCGGCAGC | 10R | GGTGTTGTGGCGGCAGC | 8R2_Bt1 | GAAATCTGAAGCGGGTGTGG | 11R | CCGCAGGTATGTTGGGC |
| 11F | CAACAACCCTACCGTGG | 11F | CAACAACCCTACCGTGG | 11F | CAACAACCCTACCGTGG | 9F | GTGTGCGTCAGATTCGG | 12F | CACCGCCCATCACCGCAA |
| 11R | CCGCAGGTATGTTGGGC | 11R | CCGCAGGTATGTTGGGC | 11R | CCGCAGGTATGTTGGGC | 9R | GAAGAAGTGGGCTCGGAC | 12R | AGTGTGCCATTGCCGTAGG |
| 12F | CACCGCCCATCACCGCAA | 12F | CACCGCCCATCACCGCAA | 12F | CACCGCCCATCACCGCAA | 10F | CCTTCACCATTCACGCCATA | 13F | CACCGCTTGAACATCG |
| 12R | CCTACGGCAATGGCACACT | 12R | CCTACGGCAATGGCACACT | 12R | CCTACGGCAATGGCACACT | 10R | GGTGTTGTGGCGGCAGC | 13R | AAGTGGCGATGGCAATGTG |
| 13F | CACCGCTTGAACATCG | 13F | CACCGCTTGAACATCG | 13F | CACCGCTTGAACATCG | 11F | CAACAACCCTACCGTGG | 14F | CCTTGGTTGGTTTCAT |
| 13R | AAGTGGCGATGGCAATGTG | 13R | AAGTGGCGATGGCAATGTG | 13R | AAGTGGCGATGGCAATGTG | 11R | CCGCAGGTATGTTGGGC | 14R | CTACTACGAAGATGAGCAC |
| 14F | CCTTGGTTGGTTTCAT | 14F | CCTTGGTTGGTTTCAT | 14F | CCTTGGTTGGTTTCAT | 12F | CACCGCCCATCACCGCAA | ||
| 14R | CTACTACGAAGATGAGCAC | 14R | CTACTACGAAGATGAGCAC | 14R | CTACTACGAAGATGAGCAC | 12R | CCTACGGCAATGGCACACT | ||
| 13F | CACCGCTTGAACATCG | ||||||||
| 13R | AAGTGGCGATGGCAATGTG | ||||||||
| 14F | CCTTGGTTGGTTTCAT | ||||||||
| 14R | CTACTACGAAGATGAGCAC | ||||||||
a
C, rtxA1 copy located on a chromosome; P, rtxA1 copy located on a plasmid.