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PFGE of NotI digestion of OG1RF and its mutant (TX5599), isogenic for the deletion from fsrC to EF_1841. Digestion with NotI showed the band corresponding to the fsrC-EF_1841 region migrating as ca. 280 kb for OG1RF and ca. 240 kb for TX5599, a difference corresponding to the 37-kb deletion in OG1RF. The band corresponding to the fsrC-EF_1841 region and the fragment after the deletion area is shown by a black arrowhead.
