TABLE 1.
Sequenced strains analyzed in this studyb
Genotype | Straina | MLST | Relevant characteristic |
Serverc | Reference(s) | |||||
---|---|---|---|---|---|---|---|---|---|---|
fsrB | gelE | Gel+ | 23.9-kb region | 14.8-kb region | efaB5 | |||||
A | V583 | 6 | + | + | + | + | − | + | TIGR | 35, 42 |
Merz96 | 103 | + | + | + | + | − | + | BI | 34 | |
TX0104 | 2 | + | + | + | + | − | + | BCM | This study | |
HH22 | 6 | + | + | + | + | − | + | BCM | 27 | |
A′ | CH188 | 9 | + | + | − | + | − | + | BI | 19, 39 |
B′ | ATCC 4200 | 105 | − | + | − | − | − | + | BI | 23 |
B | HIP11704 | 4 | − | + | − | − | − | + | BI | 24 |
T2 | 11 | − | + | − | − | − | + | BI | 20 | |
T8 | 8 | − | + | − | − | − | + | BI | 20 | |
JH2-2 | 8 | − | + | − | − | − | + | 15 | ||
TX1322 | 64 | − | + | − | − | − | + | BCM | This study | |
C | OG1RF | 1 | + | + | + | + | + | − | BCM | 8, 14 |
T1 | 21 | + | + | + | + | + | − | BI | 20 | |
T3 | 67 | + | + | + | + | + | − | BI | 20 | |
C′ | E1Sol | 93 | + | + | + | + | + | − | BI | 12 |
T11 | 65 | + | + | + | + | + | − | BI | 20 | |
D | Fly1 | 101 | + | + | + | + | P | − | BI | 23 |
E | JH1 | 40 | + | + | − | + | − | − | BI | 2, 15 |
F | ARO1/DG | 108 | + | + | + | P | − | − | BI | 22 |
G | X98 | 19 | + | + | − | − | − | − | BI | 5, 43 |
H | D6 | 16 | − | − | − | P | − | − | BI | 23 |
I | ATCC 29200 | 21 | − | P | − | P | − | − | BCM | 1 |
—d | TX5599 | 1 | − | + | − | − | − | − | This study |
The strains are listed by the genotype of the EF_1814-to-EF_1902 region. The three genotypes (A, B, and C) encompassing the greatest number of strains are listed first, followed by the genotypes with one strain each. Variations of genotypes A, B, and C were detected in the strains CH188, ATCC 4200, and E1Sol and T11 and designated A′, B′, or C′, respectively. Sequences of these isolates were analyzed for the fsrC-EF_1841 region (23.9 kb), the 14.8-kb insertion region described in OG1RF, and the putative integrative conjugative element mobile element efaB5. Strains with genotype A (V583, Merz96, TX0104, and HH22) are within CC2.
Information on the MLST and the presence (+) or absence (−) of fsrB (EF_1820), gelE (EF_1818), and gelatinase production (Gel+) was either acquired from the literature (23) and confirmed or tested by us. A “P” indicates the presence of the part of the locus/gene mentioned.
TIGR, the Institute for Genomic Research; BI, Broad Institute; BCM, Baylor College of Medicine.
TX5599 is the mutant constructed in this study by deleting 37 kb (the 23.9-kb region and the 14.8-kb region) in OG1RF.