TABLE 2.
Characteristics of four putative β-galactosidases in C. perfringens strain 13
| β-Galactosidase | % sequence similarity to: |
Other bacteria with homology | Distinguishing characteristic(s) | |||
|---|---|---|---|---|---|---|
| BgaL (CPE0771) | BgaM (CPE0831) | Pbg (CPE0167) | CPE1266 | |||
| BgaL (CPE0771) | 17.3 | 10.4 | 15 | Firmicutes | Homologous proteins often annotated as glycoside hydrolases | |
| BgaM (CPE0831) | 17.3 | 10.2 | 24.2 | Agrobacterium-Sinorhizobium | None | |
| Pbg (CPE0167) | 10.4 | 10.2 | 10.2 | Firmicutes | Transcription shown to be induced by lactose (13) and cleaved X-Gal when cloned into an E. coli plasmid and activated by ONPG (24) | |
| CPE1266 | 15 | 24.2 | 10.2 | Bifidobacterium and pathogenic members of the Firmicutes | Potential secretion signal sequence at the N terminus; homologous proteins are surface anchored and contribute to avoidance of phagocytosis by inhibiting complement deposition on the surface of the bacterium (4) | |