TABLE 2.
Cleavage of prenisin with engineered peptidase sites exported out of L. lactis containing pIL3BTCa
| Peptidase | Prenisin with engineered cleavage siteb | Dehydrations | Cleavage |
|---|---|---|---|
| NisP | MSTKDFNLDLVSVSKKDSGASPR↓ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 8 | + |
| NisP | MSTKDFNLDLVSVSKKDSGVSLR↓ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 8 | + |
| NisP | MSTKDFNLDLVSVSKKDSGVSLR↓QPSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | Incomplete | + |
| Thrombin | MSTKDFNLDLVSVSKKDSGAVPR↓ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 8 | + |
| Thrombin | MSTKDFNLDLVSVSKKDSGLVPR↓GSSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 7 | + |
| Enterokinase | MSTKDFNLDLVSVSKKDSGDDDK↓ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 8 | + |
| Enterokinase | MSTKDFNLDLVSVSKKDSDDDDK↓ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 8 | + |
| Glu-C | MSTKDFNLDLVSVSKKDSGASPD↓ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 8 | − |
| Glu-C | MSTKDFNLDLVSVSKKDSGASPE↓ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 8 | + |
| Factor Xa | MSTKDFNLDLVSVSKKDSGIDGR↓ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 8 | ± |
| Factor Xa | MSTKDFNLDLVSVSKKDSGIEGR↓ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK | 8 | ± |
a
Peptides in the supernatant were identified by MALDI-TOF mass spectrometry.
b
Substituted amino acids are indicated in boldface type.