Table I. LSFdn-induced apoptosis reduces the percentage of 3T3 cells in S + G2 phases of the cell cycle.
Samplea | Time (h) | DNA content (range of channel numbers) |
||
---|---|---|---|---|
Sub-G1 (0–124) | G1 (125–242) | S ± G2 (243–558) | ||
Control | 0 | 3.2 | 86 | 10 |
LSFdn | 0 | 3.5 | 85 | 11 |
Control | 16 | 0.6 | 55 | 44 |
LSFdn | 16 | 0.9 | 59 | 40 |
Control + 20 µM thymidine | 16 | 0.7 | 56 | 43 |
LSFdn + 20 µM thymidine | 16 | 1.5 | 66 | 33 |
Control | 22 | 0.8 | 33 | 66 |
LSFdn | 22 | 30 | 33 | 37 |
Control + 20 µM thymidine | 22 | 1.0 | 28 | 71 |
LSFdn + 20 µM thymidine | 22 | 1.3 | 29 | 70 |
aCorresponding to the histograms in Figure 4A.