Western blot analysis to evaluate the expression of the mutant CDA Y33G in presence of low molecular weight compounds in the culture medium.
A) DMSO 0%, 3%, 5%, 6%, 7%; B) Glycerol 0%, 0.5%, 1%, 5%, 10%, wt: wild-type CDA (positive control); st: Bio-Rad low molecular weight standard (97.4 kDa, phosphorylase b; 66.2 kDa, bovine serum albumin; 45.0 kDa, ovalbumin; 31.0 kDa, carbonic anhydrase; 21.5 kDa, soybean trypsin inhibitor; 14.4 kDa, lysozyme).