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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Int J Biol Macromol. 2010 Jul 14;47(4):471–482. doi: 10.1016/j.ijbiomac.2010.07.001

Figure 3.

Figure 3

A) 15% PAGE followed by western blot analysis of Y33G at different SDS concentrations. Each lane represents separate gels with different concentrations of the denaturing agent. The SDS/enzyme molar ratios in the individual lanes were as follows: lane 1, 70; lane 2, 120; lane 3, 300. St, Bio-Rad low molecular weight standard.

B) Percentage of enzymatic activity of wild-type CDA (●) andY33G mutant CDA (■) after incubations with SDS concentrations ranging from 0.35 to 1.73 mM in order to give different SDS/enzyme molar ratios. The data of wild type CDA are from [20].