Table 1.
Specific activities of Y33G mutant CDA expressed in E.coli strain after addition of chemical chaperones or specific CDA ligands (substrates and inhibitors) in the growth medium. In bold are evidenced the molecules that increase Y33G specific activity.
| Chaperons or CDA ligands | CDA Specific activity (mU/mg) | |
|---|---|---|
| No Chaperons | 0.00 | |
| DMSO | 0.50% | 0.00 |
| DMSO | 1.00 % | 3.90 |
| DMSO | 5.00% | 4.60 |
| DMSO | 6.00% | 7.00 |
| DMSO | 7.00% | 5.20 |
| Glycerol | 2.00% | 0.00 |
| Glycerol | 4.00% | 1.40 |
| Glycerol | 6.00% | 1.60 |
| Glycerol | 8.00% | 3.10 |
| Glycerol | 10.0% | 4.30 |
| Cytidine | 1.00 mM | 0.00 |
| Cytidine | 5.00 mM | 0.00 |
| Cytidine | 10.0 mM | 12.0 |
| Cytidine | 20.0 mM | 19.6 |
| Cytidine | 50.0 mM | 36.2 |
| Uridine | 0.10 mM | 9.00 |
| Uridine | 0.50 mM | 9.80 |
| Uridine | 1.00 mM | 20.0 |
| Uridine | 10.0 mM | 7.90 |
| Uridine | 50.0 mM | 9.80 |
| 5-FZEB | 0.001 mM | 0.00 |
| 5-FZEB | 0.01 mM | 15.0 |
| 5-FZEB | 0.10 mM | 11.60 |