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. Author manuscript; available in PMC: 2011 Nov 1.

Published in final edited form as: Int J Biol Macromol. 2010 Jul 14;47(4):471–482. doi: 10.1016/j.ijbiomac.2010.07.001

Table 2.

Kinetic parameters for substrates of wild-type and mutant Y33G CDA

Substrates	wild-type CDA^a	Y33G
Cytidine
Km (μM)	39.0	74.5
Vmax (U/mg)	68.3	0.152
Vmax/Km	1.7	2.04×10⁻³
Deoxycytidine
Km (μM)	39.0	47.6
Vmax (U/mg)	45.0	0.127
Vmax/Km	1.2	2.67×10⁻³
Cytosine arabinoside
Km (μM)	110.0	It is not substrate
Vmax (U/mg)	42.0
Vmax/Km	0.4
5-Azadeoxycytidine ^b
Km (μM)	74.8	349.0
Vmax (U/mg)	31.0	0.174
Vmax/Km	0.4	4.50×10⁻⁴

^a)

[17].

^b)

The wavelength used for 5-azadeoxycytidine assay was 270 nm.