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. 2001 Feb 13;98(5):2723–2728. doi: 10.1073/pnas.051629998

Figure 2.

Figure 2

Apicoplast-targeting signal in T. gondii ACC. Amino acid sequence comparison of the N terminus of ACC1 and ACC2 of T. gondii with those of cytosolic and plastid multidomain ACCs from other species (A). The first methionine residues of each ORF are underlined. Asterisks indicate the first conserved amino acid residues of the BC domain. The putative transmembrane helix-like motif (amino acids 85–107) and a signal peptide cleavage site (between A106 and G107) found in T. gondii ACC1 are double-underlined. (B) Structure of constructs for the expression of the putative ACC signal/transit peptide–GFP fusions in T. gondii. ptub, promoter of the T. gondii TUB1 gene; sag-CAT, selectable marker (chloramphenicol resistance) for T. gondii transformation; M, first methionine of ACC ORF.