Table 1.
cDNA | Primers for cDNA synthesis | Forward PCR primers | Reverse PCR primers |
---|---|---|---|
ACC1 | |||
Fragment I | Random hexamers | GTGCGTAGTGGGTTTTTGTCC | GCTGCCATGCCGTTGTTGGCGATG |
Fragment II | CAGGTGCATTTTGACTTCGATTT | TTCAGGCGTCAACCACGGAATCGA | AGAGCGAGAGTCCCCAACACATCG |
Fragment III | CAGGTGCATTTTGACTTCGATTT | GGTAGAAGCGATGAAGATGATAATG | GAACGTGGACTGCGGCCAGGCGGA |
Fragment IV | Random hexamers | ATCGATCTGCTCTCCCACTT | CAGGTGCATTTTGACTTCGATTT |
ACC2 | |||
Fragment I | AATGAGATGCAGATTGGCGTAGTT | TCCTGCGTGTGTGTTCGG | AGTCTGCTTCAGCAATAAATTCAG |
and AGATTGGCGTAGTTGTTCGAGTTC | |||
Fragment II | CCGATTCTTCCGCTTCAGAG | ATGCTTCCTATCTCGGAGGC | GTGGCTTCGCTGCAAAGAC |
Fragment III | TGTTCCCATCTGAAAGGTGAC | TTTTCCCGGGTCCCTCACCCAATGCC | CGTCGTTGCCTATCAGAATGA |
and CGTCGTTGCCTATCAGAATGA |