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. 2003 Aug;9(8):943–948. doi: 10.3201/eid0908.020748

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Phylogenetic tree for the complete 18S rRNA gene from selected Babesia spp. The tree was computed by using the quarter puzzling maximum likelihood method of the TREE-PUZZLE program and was oriented by using Theileria annulata as the outgroup. Numbers at the nodes indicate the quartet puzzling support for each internal branch. Scale bar indicates an evolutionary distance of 0.01 nucleotides per position in the sequence. Vertical distances are for clarity only. The GenBank accession numbers for the sequences used in the analysis are as follows: Babesia bigemina A, X59604; B. bovis, L19077; B. caballi, Z15104; B. divergens (Purnell isolate [12]), AY046576; B. gibsoni (genotype Asia 1), AF175300; B. odocoilei (Brushy Creek and Engeling isolates [14]), AY046577; Babesia sp. (isolated from Bos taurus), U09834; EU1 (the etiologic agent of infection in the two cases described here), AY046575; and Theileria annulata, M64243.