Fig. 2. PDI interacts specifically with Ero1-Lα. Approximately 2 × 10⁶ CHO DUKX cells were semi-permeabilized and incubated at 30°C in an in vitro translation reaction for the times indicated. Cell pellets were lysed in 1% CHAPS and the clarified lysate was split into two portions for direct analysis by SDS–PAGE (A) or immunoprecipitation with anti-PDI serum (B) prior to analysis by non-reducing (lanes 1–5) and reducing (lanes 6–10) 7.5% SDS–PAGE. Anti-PDI immunoprecipitation selectively recovered glycosylated Ero1-Lα in a complex (*).