Figure 3.

Characterization of MIP-1α and MIP-1β polymers in solution. (A) SEC of 1 mg/ml MIP-1α, MIP-1β and MIP-1α D27A with or without 0.1 mg/ml heparin. SEC was performed using phosphate-buffered saline (PBS). (B) Experimental scattering curves for 1 mg/ml MIP-1α, MIP-1β and MIP-1α D27A with or without 0.1 mg/ml heparin in PBS. Insets: cross-section, Guinier rod plots. (C) Schematic representation of our model for the polymerization process. The detailed mathematical modelling is appended in the Supplementary Material. (D) Fitting of the experimental data (solid line) with the calculated scattering from either the best polymer distribution given by our model (dashed line) or the best single polymer (dotted line) with difference between the experimental and fitted theoretical curves shown on bottom. Inset: size distribution of the polymers length distribution. The fitting errors I₀ × I_theo^a(q)−I_exp(q)+I_b are represented in the deviation curve. Parameter values were chosen to minimize the fit function , as described in the Supplementary Material.