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. 2010 Oct 15;29(23):4035–4047. doi: 10.1038/emboj.2010.258

Figure 1.

Figure 1

Mitochondria accumulate under the IS independently of TCR triggering. (A) Jurkat T cells stably expressing the mitochondrial marker mtRFP were incubated with SEE-loaded or unloaded B cells for 15 min. Images of mtRFP fluorescence from randomly selected conjugates (at least 180 for each condition, out of five independent experiments) were acquired, classified and analysed statistically as described in Materials and methods. Representative images of T cells with mitochondria recruited or not to the IS are shown in Supplementary Figure S1. Graph shows the quantitative analysis of mitochondria accumulation at the IS. Fluorescence (z projection of the stacks) and DIC representative images are shown. mtRFP was colour-coded red; α-tubulin, green. Bars, 10 μm. (B, C) Jurkat T cells expressing the mitochondrial marker mtRFP were plated with SEE-pulsed (B) or unpulsed (C) B cells and monitored by time-lapse microscopy for mitochondria relocation. Representative images taken from the digital movies (Supplementary Videos 2 and 3) at the indicated times are shown. Bars, 10 μm.