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FoF1 nanodiscs (n-FoF1) in single-molecule rotation studies. (A) Microscope slide bound n-FoF1 attached via β-subunit N-terminus 6 × His tags attached to an avidin-coated 77 × 39 nm2 nanorod via a biotinylated subunit-c cys. (B, C) Microscope fields-of-view of gold nanorods (red and green dots) bound to a slide coated with n-FoF1 in which subunit-c contained (B) or lacked (C) the cys insertion mutation.
