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. 2010 Oct 15;29(23):4048–4061. doi: 10.1038/emboj.2010.257

Figure 2.

Figure 2

Bach2-deficient B cells show precocious differentiation to antibody-secreting cell. (A) CFSE labelled splenic B cells of Bach2+/+ (left) or Bach2−/− (right) mice were stimulated with LPS for 3 days and were analysed for their expression of CD138 by using FACS. The profiles of cell division and acquisition of surface CD138 are shown by boxes (upper panels). CFSE intensity histograms of the splenic B-cell culture are shown in lower panels. FACS data are representative of three independent experiments. (B) The percentage of cells in each division expressing CD138. FACS data of Bach2+/+ (open circle) and Bach2−/− (closed triangle) B cells are expressed as the mean±s.e. of triplicate cultures. (C) Splenic B cells of Bach2+/+ (upper panels) and Bach2−/− (lower panels) mice were harvested at 20 h after LPS stimulation and assayed for IgM secretion by ELISPOT. Representative ELISPOT wells (left) and the proportion of cell-secreting IgM (right) are shown from two independent experiments.