Table 1.
Bacterial strains used for inclusivity and exclusivity testing of the Mptb52.16 real-time PCR assay
| Genus and species | Source |
|---|---|
|
Mycobacterium avium subsp. paratuberculosis |
CIPa 103964 |
|
Mycobacterium avium subsp. paratuberculosis |
CIP 103967 |
|
Mycobacterium avium subsp. paratuberculosis |
CIP 103968 |
|
Mycobacterium avium subsp. paratuberculosis |
CIP 103971 |
|
Mycobacterium avium subsp. paratuberculosis |
CIP 103972 |
|
Mycobacterium avium subsp. paratuberculosis |
CIP 103973 |
|
Mycobacterium avium subsp. paratuberculosis |
CIP 103974 |
|
Mycobacterium avium subsp. paratuberculosis |
CIP 103975 |
|
Mycobacterium avium subsp. paratuberculosis |
CIP 103976 |
|
Mycobacterium avium subsp. paratuberculosis |
CIP 103977 |
|
Mycobacterium abscessus |
DSMb 44196 |
|
Mycobacterium aurum |
DSM 43999 |
|
Mycobacterium avium subsp.avium |
DSM 44156 |
|
Mycobacterium avium subsp.silvaticum |
DSM 44175 |
|
Mycobacterium chitae |
DSM 44633 |
|
Mycobacterium duvalii |
DSM 44244 |
|
Mycobacterium flavescens |
DSM 43991 |
|
Mycobacterium fortuitum subsp. fortuitum |
DSM 46621 |
|
Mycobacterium gordonae |
DSM 44160 |
|
Mycobacterium intracellulare |
DSM 43223 |
|
Mycobacterium kansasii |
DSM 44162 |
|
Mycobacterium marinum |
DSM 44344 |
|
Mycobacterium neoaurum |
DSM 44074 |
|
Mycobacterium nonchromogenicum |
DSM 44164 |
|
Mycobacterium parafortuitum |
DSM 43528 |
|
Mycobacterium phlei |
DSM 43239 |
|
Mycobacterium scrofulaceum |
DSM 43992 |
|
Mycobacterium shiumoidei |
DSM 44152 |
|
Mycobacterium thermoresistibile |
DSM 44167 |
|
Mycobacterium triviale |
DSM 44153 |
|
Mycobacterium vaccae |
DSM 43292 |
|
Mycobacterium xenopi |
DSM 43995 |
|
Bacillus cereus |
NCTCc 7464 |
|
Clostridium bovis |
DSM 20582 |
|
Clostridium pyogenes |
DSM 20630 |
|
Enterococcus faecalis |
ATCCd 19433 |
|
Escherichia coli |
NCTC 9001 |
|
Lactococcus lactis |
DSM 20069 |
|
Listeria monocytogenes |
NCTC 11994 |
|
Pseudomonas aeruginosa |
NCTC 10662 |
|
Salmonella typhimurium |
ATCC 14028 |
|
Staphylococcus aureus |
NCTC 1803 |
|
Staphylococcus epidermidis |
ATCC 12228 |
|
Streptococcus agalactiae |
DSM 2134 |
|
Streptococcus dysgalactiae subsp. dysgalactiae |
DSM 20662 |
|
Streptococcus dysgalactiae subsp. equisimilis |
DSM 6176 |
|
Streptococcus thermophilus |
DSM 20617 |
| Streptococcus uberis | DSM 20569 |
Colonies (MAP and non-MAP mycobacteria) suspended in 1 ml of Ringer's solution or overnight broth cultures (raw milk microflora) were subjected to DNA isolation using the NucleoSpin tissue kit (Machery-Nagel, Düren, Germany). The DNA concentration was measured fluorimetrically using a HoeferDyNA Quant200 apparatus (Pharmacia Biotech, San Francisco, USA) and the DNA was diluted to 0.1 ng/μl ddH2O.
Notes: a CIP: Institute Pasteur, b DSM: German Collection of Microorgansims, c NCTC: National Collection of Type Cultures, d ATCC: American Type Culture Collection