Table 1.
Rate of polymerization and processivities obtained from ensemble and single-molecule measurements:
| Ensemble studies | Single-molecule studies | |||
|---|---|---|---|---|
| Protein Complex | Rate of reaction (nt/s) | Processivity (kb) | Rate of reaction (nt/s) | Processivity (kb) |
| Phage T7: | ||||
| Gp4 | 15 [86] | 0.06 [86] | 29 (5.2 pN) [85] | 0.343 (5.2 pN) [85] |
| Gp5 | - | 0.015 [93] | 210 (exo-) [48] | - |
| Gp5+Trx | 110 [93] | 10 [93] | 100 [47] | 0.42 (15 pN) [47] |
| Gp5+Trx+gp4 | 120 [92] | - | 164 [52] | 17 [52] |
| Gp5+Trx+gp4+gp2.5 | 300 [105] | 10 [105] | 75 (22 °C) [61] 141 (37 °C)[61] |
25 ± 17 [61] 51 ± 9 [61] |
| Phage T4: | ||||
| Gp41 | 30 [106] | 0.65 [107] | 30 [49] | 0.8 [49] |
| Gp43 | 250 [108] | 0.85 [108] | - | - |
| Gp43+gp45 | 600 [108, 109] | 3 [108, 109] | - | - |
| Gp43+gp45+gp41+gp61+gp32 | 250 [108, 109] | > 20 [109] | - | - |
| E. coli: | ||||
| DnaB | 291 [110] | - | - | - |
| Klenow Fragment | 50 [67] | 0.188 [67] | 13 [48] | - |
| Pol III | 20 [27] | 0.01 [27] | - | - |
| Pol III + β | 750 [102, 103] | 1 [103] | 347 [51] | 1.40 [51] |
| Pol III+β+DnaB | - | - | 417 [51] | 10.5 [51] |
| Pol III + β+DnaG | - | - | 340 [51] | 2.9 [51] |
| Pol III+β+DnaB+SSB | 750 [101, 102] | >50 [101, 102] | 535 [61] | 85 [61] |