Fig. 2. The R468E and T535R mutations in the NFAT1 DBD strongly influence the interaction of NFAT1 with Fos and Jun. (A) Jun homodimers. (B) Fos–Jun heterodimers. EMSAs were performed with the murine IL-2 promoter ARRE2 probe and wild-type (left panels), R468E (middle panels) or T535R (right panels) recombinant NFAT1 DBDs. Right panels show results of densitometric quantification.