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. 2010 Sep 20;28(31):4674–4682. doi: 10.1200/JCO.2010.28.5064

Fig 3.

Fig 3.

(A) Relative T-cell leukemia 1A (TCL1A) expression in U2OS cells transfected with estrogen receptor alpha (ERα) exposed to 0.1 nmol (nM)/L 17-β-estradiol over 24 hours. (B) Relative TCL1A expression in U2OS cells transfected with ER beta (ERβ) exposed to 0.1 nmol/L 17-β-estradiol over 24 hours. (C) Chromatin immunoprecipitation assay using ERα-transfected lymphoblastoid cells with known genotype for the rs11849538 single nucleotide polymorphism (SNP). DNA ladder 1 (LAD1) and DNA ladder 2 (LAD2) are both Invitrogen (Carlsbad, CA) 1-kb DNA ladders, with LAD2 being a 1-kb Plus DNA ladder. Lanes 2 to 5 are polymerase chain reaction (PCR) products from DNA that was bound to human ERα antibody (Ab). The inputs for the variant (V) and wild type (WT) were PCR amplification products of pools of sheared DNA from the entire genome. (D) SNP-related differences in TCL1A expression and estrogen response in nine V (rs11849538) and nine WT lymphoblastoid cell lines transfected with ERα. (*) P < .05. (†) P < .01.