FIGURE 6.

Effects of siRNA-mediated suppression of ARNT/HIF-1β on key genes involved in the metabolic regulation of β-cell function in 832/13 cells. Gene expression is expressed as a percentage of the target gene from siControl-treated cells and corrected for by an internal control gene cyclophilin E (Cyp). There was no significant difference seen between treatment groups for cyclophilin E (n = 5). HNF4a, hepatocyte nuclear factor-4α; HNF1a, hepatocyte nuclear factor-1α; HIF1a, hypoxia-inducible factor 1α; GK, glucokinase; GLUT2, glucose transporter-2; PLGγ1, phospholipase γ-1; PC, pyruvate carboxylase; PDHα1, pyruvate dehydrogenase (α1 subunit); MEc, cytosolic malic enzyme; ICDc, cytosolic isocitrate dehydrogenase; CIC, citrate carrier; DIC, dicarboxylate carrier; OGC, α-ketoglutarate carrier; FAS, fatty-acid synthase; CPT1a, carnitine palmitoyltransferase 1α. *, p < 0.05; **, p < 0.01; ***, p < 0.001 siControl versus siANRT1.