FIGURE 7.

Biphasic insulin secretion in pseudoislets 72 h after transfection with either siRNA control (siControl) or siRNA against ARNT/HIF-1β (siARNT1). A, pseudoislets were perifused from 1 to 8 min with 2.8 mm low glucose (LG) followed by 16.7 mm high glucose (time points from 9–40 min) and finally with 16.7 mm high glucose with 40 mm KCl (time points from 41–60 min) (n = 5). B, area under the curve of the insulin secretion responses in A (basal, 1–8 min; 1st phase, 9–18 min; 2nd phase, 19–40 min; HG + KCl, 41–60 min). **, p < 0.01; ***, p < 0.001 siControl versus siANRT1.