FIGURE 11.

RNA silencing of Sp1, Sp3, and N-Myc modestly reduces serine-exchange activity. Rat astrocytes were transfected with DsiRNA duplexes targeting rat Sp1, Sp3, or N-Myc or with non-targeting 27-mer RNA duplex (NegCtrl). After 96 h cell lysates were prepared and serine-exchange activity was measured. White bars (no addition), total PSS activity; gray bars (+50 mm choline), PSS2 activity. Data are mean ± S.D. of triplicate analyses from one of three experiments with similar results. *, p < 0.05 versus NegCtrl.