FIGURE 2.
Transcriptional regulation of Pss1 in brain. A, binding of nuclear proteins from mouse tissues to the −356/−150-bp fragment. Nuclear extracts were isolated from brain (B), liver (L), heart (H), and kidney (K) of neonatal mice. 20 μg of protein were used in EMSAs with 32P-labeled probes: −356/−302 bp, −308/−250 bp, −260/−198 bp, and −213/−150 bp. Protein-DNA complexes (arrows) were resolved by 6% non-denaturing polyacrylamide gel electrophoresis and detected by autoradiography. Lanes indicated by − lacked nuclear extract. Data are representative of three to four independent experiments with similar results. B, Pss1 transcript levels in neonatal mouse tissues were quantified relative to reference genes (cyclophilin A, CypA; β-actin, ActB; phosphoribosyltransferase-1, Hprt1) by real time qPCR and normalized using geNorm software.