Skip to main content
. 2010 Nov 10;286(2):1061–1073. doi: 10.1074/jbc.M110.158709

FIGURE 8.

FIGURE 8.

Functional binding of transcription factors assessed by ChIP. A, schematic representation of the −387/−142-bp region of the promoter (ChIP_387/142Pss1) amplified with primers flanking Sp, Myc, and E47-Tal1 binding sites. B, input sample and immunoprecipitation (IP) product with rabbit preimmune serum (IgG; negative control) or with antibodies against N-Myc, Sp1, Sp3, Sp4, E47, Tal1, or YY1 (a non-relevant antibody) performed on cross-linked chromatin from mouse astrocytes and amplified either with ChIP_F (forward, −385 relative to transcriptional start site) and ChIP_R (reverse, −142 relative to transcriptional start site) primers or with primers amplifying a region of the mouse phosphatidylethanolamine N-methyltransferase gene (ChIP_NegCtrl) as negative control at DNA level. Ld, DNA ladder.