FIGURE 2.

IFNλ-dependent activation of RSK1 and mTOR pathways in ARPE-19 cells. A–C, serum-starved ARPE-19 cells were pretreated with rapamycin or U0126 as indicated and then treated with IFNλ for the indicated times. Total cell lysates were resolved by SDS-PAGE and immunoblotted with anti-phospho-Thr³⁵⁹/Ser³⁶³ RSK1, anti-RSK1, anti-phospho-Ser⁴²²-eIF4B, or anti-eIF4B antibodies (A); with anti-phospho-Thr^37/46-4E-BP1 or anti-GAPDH antibodies (B); or with antibodies against the phosphorylated form of p70S6K on Thr⁴²¹/Ser⁴²⁴ or against p70S6K or anti-GAPDH (C), as indicated.