FIGURE 2.

Assessment of the effect of hepatic PPARδ expression on glycogen synthesis and lipogenesis in chow-fed mice. A and B, histological, glycogen, and lipid analyses of liver samples from GFP and PPARδ adenovirus-injected mice on a chow diet. Liver samples were collected from ad libitum fed animals 2 weeks following virus injection. Hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) (counterstained with hematoxylin) staining (A) as well as enzymatic assays (B) were conducted to determine glycogen and triglyceride content. C, levels of liver glycogen synthase (GS) and ACC determined by Western blotting. The samples were collected from four individual animals from GFP and PPARδ adenovirus-injected mice. Actin was included as the loading control. D, hepatic gene expression determined by real time qPCR. Liver samples were harvested from control (GFP) or adPPARδ (PPARδ) mice under ad libitum feeding condition. *, p < 0.05.