FIGURE 7.

α₂M·PSA-induced up-regulation of PSA, GRP78 phospho-MEK1/2, phospho-ERK1/2, phospho-p38 MAPK, phospho-Akt^Thr-308, phospho-Akt^Ser-473, and phospho-S6K in DU-145 prostate cancer cells. A–G, treatments of cells in each panel are as follows: buffer (lane 1), native α₂M (50 pm for 20 min) (lane 2), α₂M·methylamine (50 pm for 20 min) (lane 3), premade α₂M·PSA (50 pm for 20 min) (lane 4), and α₂M·PSA made in culture (5 μg/ml for 20 min) (lane 5). □, phospho-Akt^Thr-308; ■, phospho-Akt^Ser-473. Changes in each immunoblot are shown in fluorescent units (× 10³) as determined by a PhosphorImager and are expressed as the mean ± S.E. from three independent experiments, each assayed in triplicate in the bar diagrams above the respective immunoblot. The protein loading controls (unphosphorylated protein or actin) are shown below the respective immunoblot. Values significantly different at the 5% level from buffer- and native α₂M-treated cells are marked with an asterisk.