FIGURE 8.

Effect of α₂M·PSA on levels of PSA, GRP78, phospho-MEK1/2, phospho-ERK1/2, phospho-p38 MAPK, phospho-Akt^Thr-308, phospho-Akt^Ser-473, and phospho-S6K in PC-3 cancer cells. A–G, treatments of cells in each panel are as follows: buffer (lane 1), native α₂M (50 pm for 20 min) (lane 2), α₂M·methylamine (50 pm for 20 min) (lane 3), premade α₂M·PSA (50 pm for 20 min) (lane 4), and α₂M·PSA made in culture (5 μg/ml for 20 min (lane 5). □, phospho-Akt^Thr-308; ■, phospho-Akt^Ser-473. Changes in each immunoblot are shown in fluorescence units (× 10³) as determined by a PhosphorImager and are expressed as the mean ± S.E. from three independent experiments assayed in triplicate in the bar diagram above the respective immunoblot. The protein loading controls (unphosphorylated protein or actin) are shown below the respective immunoblot.