FIGURE 4.

Inhibition of intein splicing in E. coli using the TS reporter. Cell growth was measured at various cisplatin concentrations by optical density at 600 nm in the absence of thymine (blue lines) and in the presence of thymine (red lines). The thyA (TS⁻) E. coli host was transformed with the plasmids pK-TS (○), pK-TS-I₄₄₀ (□), or pK-TS-I₁₆₈ (▵). The calculated IC₅₀ is 7.8 μm. Error bars correspond to the standard deviations.