FIGURE 6.

Intein overexpression can mitigate sensitivity to cisplatin. A, intein-mediated rescue of mycobacterial inhibition by cisplatin. M. bovis BCG with RecA intein-expression plasmid (triangle) or empty vector (square) was grown in liquid broth with kanamycin in the presence and absence of cisplatin at the concentrations shown. B, rescue is Cys1-dependent. M. bovis BCG expressing the RecA intein shows enhanced cisplatin resistance when compared with a vector control and M. bovis BCG expressing a RecA intein with the C1A mutation. Mycobacteria were grown to stationary phase in medium lacking cisplatin and then plated at a 10⁻⁶-fold dilution onto agar with the indicated concentration of cisplatin.